Comparisons of mass spectrometry compatible surfactants for global analysis of the mammalian brain proteome.

ABSTRACT: Methods for the global analysis of protein expression offer an approach to study the molecular basis of disease. Studies of protein expression in tissue, such as brain, are complicated by the need for efficient and unbiased digestion of proteins that permit identification of peptides by shotgun proteomic methods. In particular, identification and characterization of less abundant membrane proteins has been of great interest for studies of brain physiology, but often proteins of interest are of low abundance or exist in multiple isoforms. Parsing protein isoforms as a function of disease will be essential. In this study, we develop a digestion scheme using detergents compatible with mass spectrometry that improves membrane protein identification from brain tissue. We show the modified proce

SUBMITTER: Chen EI 

PROVIDER: S-EPMC2975600 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature