An incomplete TCA cycle increases survival of Salmonella Typhimurium during infection of resting and activated murine macrophages.

ABSTRACT:

Background

In comparison to the comprehensive analyses performed on virulence gene expression, regulation and action, the intracellular metabolism of Salmonella during infection is a relatively under-studied area. We investigated the role of the tricarboxylic acid (TCA) cycle in the intracellular replication of Salmonella Typhimurium in resting and activated macrophages, epithelial cells, and during infection of mice.

Methodology/principal findings

We constructed deletion mutations of 5 TCA cycle genes in S. Typhimurium including gltA, mdh, sdhCDAB, sucAB, and sucCD. We found that the mutants exhibited increased net intracellular replication in resting and activated murine macrophages compared to the wild-type. In contrast, an epithelial cell infection model showed that the

SUBMITTER: Bowden SD 

PROVIDER: S-EPMC2975626 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature