Corruption of phage display libraries by target-unrelated clones: diagnosis and countermeasures.

ABSTRACT: Phage display is used to discover peptides or proteins with a desired target property-most often, affinity for a target selector molecule. Libraries of phage clones displaying diverse surface peptides are subject to a selection process designed to enrich for the target behavior and subsequently propagated to restore phage numbers. A recurrent problem is enrichment of clones, called target-unrelated phages or peptides (TUPs), that lack the target behavior. Many TUPs are propagation related; they have mutations conferring a growth advantage and are enriched during the propagations accompanying selection. Unlike other filamentous phage libraries, fd-tet-based libraries are relatively resistant to propagation-related TUP corruption. Their minus-strand origin is disrupted by a large cassette th

SUBMITTER: Thomas WD 

PROVIDER: S-EPMC2975937 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature