Generation of Fab fragment-like molecular recognition proteins against staphylococcal enterotoxin B by phage display technology.

ABSTRACT: Antigen-binding fragments (Fab fragments) and single-chain variable fragments (scFv) against staphylococcal enterotoxin B (SEB) were produced by phage display technology. SEB epitopes were first identified by phage display approach using the commercial anti-SEB monoclonal antibody ab53981 as the target. Heptamer and dodecamer mimotope peptides recognized by ab53981 were screened from Ph.D-7 or Ph.D-12 random peptide phage libraries expressed in Escherichia coli. The isolated 7-mer and 12-mer mimotopes were shown to share a sequence homologous to ⁸PDELHK¹⁴S in the amino acid sequence of SEB. The N-terminal 15-mer peptide of SEB was determined to be an epitope of ab53981. After immunization of mice with maltose-binding protein-tagged N-terminal 15-mer peptide, a phage display Fab library was

SUBMITTER: Urushibata Y 

PROVIDER: S-EPMC2976098 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature