Impacts of inter- and intralaboratory variations on the reproducibility of microbial community analyses.

ABSTRACT: With the advent of molecular biological techniques, especially next-generation sequencing and metagenomics, the number of microbial biogeography studies is rapidly increasing. However, these studies involve the synthesis of data generated by different laboratories using different protocols, chemicals, etc., all with inherent biases. The aim of this study was to assess inter- as well as intralaboratory variations in microbial community composition when standardized protocols are applied to a single soil sample. Aliquots from a homogenized soil sample from a rice field in Italy were sent to five participating laboratories. DNA was extracted by two investigators per laboratory using an identical protocol. All DNA samples were sent to one laboratory to perform DNA quantification, quantitative

SUBMITTER: Pan Y 

PROVIDER: S-EPMC2976186 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature