Improved method for bacterial cell capture after flow cytometry cell sorting.

ABSTRACT: Fixed cells with different nucleic acid contents and scatter properties (low nucleic acid [LNA], high nucleic acid 1 [HNA1], and HNA2) were sorted by flow cytometry (FCM). For each sort, 10,000 cells were efficiently captured on poly-l-lysine-coated microplates, resulting in efficient and reproducible PCR amplification.

SUBMITTER: Guillebault D 

PROVIDER: S-EPMC2976227 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature