MRNA degradation plays a significant role in the program of gene expression regulated by phosphatidylinositol 3-kinase signaling.

ABSTRACT: Control of gene expression by the phosphatidylinositol (PI) 3-kinase/Akt pathway plays an important role in mammalian cell proliferation and survival, and numerous transcription factors and genes regulated by PI 3-kinase signaling have been identified. Because steady-state levels of mRNA are regulated by degradation as well as transcription, we have investigated the importance of mRNA degradation in controlling gene expression downstream of PI 3-kinase. We previously performed global expression analyses that identified a set of approximately 50 genes that were downregulated following inhibition of PI 3-kinase in proliferating T98G cells. By blocking transcription with actinomycin D, we found that almost 40% of these genes were regulated via effects of PI 3-kinase on mRNA stability. Analyse

SUBMITTER: Graham JR 

PROVIDER: S-EPMC2976374 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature