An experimentally derived confidence score for binary protein-protein interactions.

ABSTRACT: Information on protein-protein interactions is of central importance for many areas of biomedical research. At present no method exists to systematically and experimentally assess the quality of individual interactions reported in interaction mapping experiments. To provide a standardized confidence-scoring method that can be applied to tens of thousands of protein interactions, we have developed an interaction tool kit consisting of four complementary, high-throughput protein interaction assays. We benchmarked these assays against positive and random reference sets consisting of well documented pairs of interacting human proteins and randomly chosen protein pairs, respectively. A logistic regression model was trained using the data from these reference sets to combine the assay outputs an

SUBMITTER: Braun P 

PROVIDER: S-EPMC2976677 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature