Arbovirus detection in insect vectors by rapid, high-throughput pyrosequencing.

ABSTRACT:

Background

Despite the global threat caused by arthropod-borne viruses, there is not an efficient method for screening vector populations to detect novel viral sequences. Current viral detection and surveillance methods based on culture can be costly and time consuming and are predicated on prior knowledge of the etiologic agent, as they rely on specific oligonucleotide primers or antibodies. Therefore, these techniques may be unsuitable for situations when the causative agent of an outbreak is unknown.

Methodology/principal findings

In this study we explored the use of high-throughput pyrosequencing for surveillance of arthropod-borne RNA viruses. Dengue virus, a member of the positive strand RNA Flavivirus family that is transmitted by several members of the Aedes genus of

SUBMITTER: Bishop-Lilly KA 

PROVIDER: S-EPMC2976685 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature