Immunoproteomic analysis of human serological antibody responses to vaccination with whole-cell pertussis vaccine (WCV).

ABSTRACT:

Background

Pertussis (whooping cough) caused by Bordetella pertussis (B.p), continues to be a serious public health threat. Vaccination is the most economical and effective strategy for preventing and controlling pertussis. However, few systematic investigations of actual human immune responses to pertussis vaccines have been performed. Therefore, we utilized a combination of two-dimensional electrophoresis (2-DE), immunoblotting, and mass spectrometry to reveal the entire antigenic proteome of whole-cell pertussis vaccine (WCV) targeted by the human immune system as a first step toward evaluating the repertoire of human humoral immune responses against WCV.

Methodology/principal findings

Immunoproteomic profiling of total membrane enriched proteins and extracellular protein

SUBMITTER: Zhu YZ 

PROVIDER: S-EPMC2976700 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature