Applying harmonic optical microscopy for spatial alignment of atrial collagen fibers.

ABSTRACT:

Background

Atrial fibrosis creates a vulnerable tissue for atrial fibrillation (AF), but the spatial disarray of collagen fibers underlying atrial fibrosis is not fully elucidated.

Objective

This study hypothesizes that harmonics optical microscopy can illuminate the spatial mal-alignment of collagen fibers in AF via a layer-by-layer approach.

Patients and methods

Atrial tissues taken from patients who underwent open-heart surgery were examined by harmonics optical microscopy. Using the two-dimensional Fourier transformation method, a spectral-energy description of image texture was constituted and its entropy was used to quantify the mal-alignment of collagen fibers. The amount of collagen fiber was derived from its area ratio to total atrial tissue in each image. Se

SUBMITTER: Chiu YW 

PROVIDER: S-EPMC2976704 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature