Identification and characterization of novel polymorphisms in the basal promoter of the human transporter, MATE1.

ABSTRACT:

Objectives

Human multidrug and toxin extrusion member 1, MATE1 (SLC47A1), plays an important role in the renal and biliary excretion of endogenous and exogenous organic cations including many therapeutic drugs. In this study, we characterized the transcriptional effects of five polymorphic variants and six common haplotypes in the basal promoter region of MATE1 that were identified in 272 DNA samples from ethnically diverse US populations.

Methods

We measured luciferase activities of the six common promoter haplotypes of MATE1 using in-vitro and in-vivo reporter assays.

Results

Haplotypes that contain the most common variant (mean allele frequency in four ethnic groups: 0.322), g.-66T>C, showed a significant decrease in reporter activities compared to the reference. T

SUBMITTER: Ha Choi J 

PROVIDER: S-EPMC2976711 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature