The roles of the Saccharomyces cerevisiae RecQ helicase SGS1 in meiotic genome surveillance.

ABSTRACT:

Background

The Saccharomyces cerevisiae RecQ helicase Sgs1 is essential for mitotic and meiotic genome stability. The stage at which Sgs1 acts during meiosis is subject to debate. Cytological experiments showed that a deletion of SGS1 leads to an increase in synapsis initiation complexes and axial associations leading to the proposal that it has an early role in unwinding surplus strand invasion events. Physical studies of recombination intermediates implicate it in the dissolution of double Holliday junctions between sister chromatids.

Methodology/principal findings

In this work, we observed an increase in meiotic recombination between diverged sequences (homeologous recombination) and an increase in unequal sister chromatid events when SGS1 is deleted. The first of these o

SUBMITTER: Amin AD 

PROVIDER: S-EPMC2976770 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature