Gadonanotubes as magnetic nanolabels for stem cell detection.

ABSTRACT: Stem cell-based therapies have emerged as a promising approach in regenerative medicine. In the development of such therapies, the demand for imaging technologies that permit the noninvasive monitoring of transplanted stem cells in vivo is growing. Here, we report the performance of gadolinium-containing carbon nanocapsules, or gadonanotubes (GNTs), as a new T₁-weighted magnetic resonance imaging (MRI) intracellular labeling agent for pig bone marrow-derived mesenchymal stem cells (MSCs). Without the use of a transfection agent, micromolar concentrations of GNTs can deliver up to 10⁹ Gd(3+) ions per cell without compromising cell viability, differentiation potential, proliferation pattern, and phenotype. Imaging 10 × 10⁶ GNT-labeled MSCs demonstrates a nearly two-fold reduction in T₁ relax

SUBMITTER: Tran LA 

PROVIDER: S-EPMC2976808 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature