High-order oligomerization is required for the function of the H-NS family member MvaT in Pseudomonas aeruginosa.

ABSTRACT: H-NS is an abundant DNA-binding protein that has been implicated in the silencing of foreign DNA in several different bacteria. The ability of H-NS dimers to form higher-order oligomers is thought to aid the polymerization of the protein across AT-rich stretches of DNA and facilitate gene silencing. Although the oligomerization of H-NS from enteric bacteria has been the subject of intense investigation, little is known regarding the oligomerization of H-NS family members from bacteria outside of the enterobacteriaceae, many of which share little sequence similarity with their enteric counterparts. Here we show that MvaT, a member of the H-NS family of proteins from Pseudomonas aeruginosa, can form both dimers and higher-order oligomers, and we identify a region within MvaT that mediates hi

SUBMITTER: Castang S 

PROVIDER: S-EPMC2978250 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature