Nucleoid occlusion prevents cell division during replication fork arrest in Bacillus subtilis.

ABSTRACT: How bacteria respond to chromosome replication stress has been traditionally studied using temperature-sensitive mutants and chemical inhibitors. These methods inevitably arrest all replication and lead to induction of transcriptional responses and inhibition of cell division. Here, we used repressor proteins bound to operator arrays to generate a single stalled replication fork. These replication roadblocks impeded replisome progression on one arm, leaving replication of the other arm and re-initiation unaffected. Remarkably, despite robust generation of RecA-GFP filaments and a strong block to cell division during the roadblock, patterns of gene expression were not significantly altered. Consistent with these findings, division inhibition was not mediated by the SOS-induced regulator Yne

SUBMITTER: Bernard R 

PROVIDER: S-EPMC2978284 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature