CyDye immunoblotting for proteomics: co-detection of specific immunoreactive and total protein profiles.

ABSTRACT: The development of ECL-Plex CyDye-conjugated secondary antibodies allows the advancement of conventional Western blotting, opening up possibilities for highly sensitive and quantitative protein confirmation and identification. We report a novel proteomic method to simultaneously visualise the total protein profile as well as the specific immunodetection of an individual protein species by combining cyanine CyDye pre-labelled proteins and antibody immunoblotting. This technique proposes to revolutionise both 2-D immunoprobing and protein confirmation following MS analysis.

SUBMITTER: Donoghue PM 

PROVIDER: S-EPMC2978322 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature