Verifying expressed transcript variants by detecting and assembling stretches of consecutive exons.

ABSTRACT: We herein describe an integrated system for the high-throughput analysis of splicing events and the identification of transcript variants. The system resolves individual splicing events and elucidates transcript variants via a pipeline that combines aspects such as bioinformatic analysis, high-throughput transcript variant amplification, and high-resolution capillary electrophoresis. For the 14 369 human genes known to have transcript variants, minimal primer sets were designed to amplify all transcript variants and examine all splicing events; these have been archived in the ASprimerDB database, which is newly described herein. A high-throughput thermocycler, dubbed GenTank, was developed to simultaneously perform thousands of PCR amplifications. Following the resolution of the various am

SUBMITTER: Hsiao TH 

PROVIDER: S-EPMC2978383 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature