Systematic quantification of negative feedback mechanisms in the extracellular signal-regulated kinase (ERK) signaling network.

ABSTRACT: Cell responses are actuated by tightly controlled signal transduction pathways. Although the concept of an integrated signaling network replete with interpathway cross-talk and feedback regulation is broadly appreciated, kinetic data of the type needed to characterize such interactions in conjunction with mathematical models are lacking. In mammalian cells, the Ras/ERK pathway controls cell proliferation and other responses stimulated by growth factors, and several cross-talk and feedback mechanisms affecting its activation have been identified. In this work, we take a systematic approach to parse the magnitudes of multiple regulatory mechanisms that attenuate ERK activation through canonical (Ras-dependent) and non-canonical (PI3K-dependent) pathways. In addition to regulation of receptor

SUBMITTER: Cirit M 

PROVIDER: S-EPMC2978602 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature