Staphylococcal major autolysin (Atl) is involved in excretion of cytoplasmic proteins.

ABSTRACT: Many microorganisms excrete typical cytoplasmic proteins into the culture supernatant. As none of the classical secretion systems appears to be involved, this type of secretion was referred to as "nonclassical protein secretion." Here, we demonstrate that in Staphylococcus aureus the major autolysin plays a crucial role in release of cytoplasmic proteins. Comparative secretome analysis revealed that in the wild type S. aureus strain, 22 typical cytoplasmic proteins were excreted into the culture supernatant, although in the atl mutant they were significantly decreased. The presence or absence of prophages had little influence on the secretome pattern. In the atl mutant, secondary peptidoglycan hydrolases were increased in the secretome; the corresponding genes were transcriptionally up-reg

SUBMITTER: Pasztor L 

PROVIDER: S-EPMC2978608 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature