Replication and recombination factors contributing to recombination-dependent bypass of DNA lesions by template switch.

ABSTRACT: Damage tolerance mechanisms mediating damage-bypass and gap-filling are crucial for genome integrity. A major damage tolerance pathway involves recombination and is referred to as template switch. Template switch intermediates were visualized by 2D gel electrophoresis in the proximity of replication forks as X-shaped structures involving sister chromatid junctions. The homologous recombination factor Rad51 is required for the formation/stabilization of these intermediates, but its mode of action remains to be investigated. By using a combination of genetic and physical approaches, we show that the homologous recombination factors Rad55 and Rad57, but not Rad59, are required for the formation of template switch intermediates. The replication-proficient but recombination-defective rfa1-t11 m

SUBMITTER: Vanoli F 

PROVIDER: S-EPMC2978687 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature