Thermodynamic additivity of sequence variations: an algorithm for creating high affinity peptides without large libraries or structural information.

ABSTRACT:

Background

There is a significant need for affinity reagents with high target affinity/specificity that can be developed rapidly and inexpensively. Existing affinity reagent development approaches, including protein mutagenesis, directed evolution, and fragment-based design utilize large libraries and/or require structural information thereby adding time and expense. Until now, no systematic approach to affinity reagent development existed that could produce nanomolar affinity from small chemically synthesized peptide libraries without the aid of structural information.

Methodology/principal findings

Based on the principle of additivity, we have developed an algorithm for generating high affinity peptide ligands. In this algorithm, point-variations in a lead sequence are scr

SUBMITTER: Greving MP 

PROVIDER: S-EPMC2978705 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature