The Gag cleavage product, p12, is a functional constituent of the murine leukemia virus pre-integration complex.

ABSTRACT: The p12 protein is a cleavage product of the Gag precursor of the murine leukemia virus (MLV). Specific mutations in p12 have been described that affect early stages of infection, rendering the virus replication-defective. Such mutants showed normal generation of genomic DNA but no formation of circular forms, which are markers of nuclear entry by the viral DNA. This suggested that p12 may function in early stages of infection but the precise mechanism of p12 action is not known. To address the function and follow the intracellular localization of the wt p12 protein, we generated tagged p12 proteins in the context of a replication-competent virus, which allowed for the detection of p12 at early stages of infection by immunofluorescence. p12 was found to be distributed to discrete puncta, i

SUBMITTER: Prizan-Ravid A 

PROVIDER: S-EPMC2978732 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature