CD8+ enriched "young" tumor infiltrating lymphocytes can mediate regression of metastatic melanoma.

ABSTRACT:

Purpose

Tumor-infiltrating lymphocytes (TIL) and interleukin (IL)-2 administered following lymphodepletion can cause the durable complete regression of bulky metastatic melanoma in patients refractory to approved treatments. However, the generation of a unique tumor-reactive TIL culture for each patient may be prohibitively difficult. We therefore investigated the clinical and immunologic impact of unscreened, CD8+ enriched "young" TIL.

Experimental design

Methods were developed for generating TIL that minimized the time in culture and eliminated the individualized tumor-reactivity screening step. Thirty-three patients were treated with these CD8+ enriched young TIL and IL-2 following nonmyeloablative lymphodepletion (NMA). Twenty-three additional patients were treated with

SUBMITTER: Dudley ME 

PROVIDER: S-EPMC2978753 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature