Isolation of the regulatory regions and genomic organization of the porcine alpha1,3-galactosyltransferase gene.

ABSTRACT:

Background

Alpha1,3-galactosyltransferase (alpha1,3GT) is an enzyme that produces carbohydrate chains termed alphaGal epitopes found in most mammals, although some species of higher primates, including human, are notable exceptions. The evolutionary origin of the lost alpha1,3GT enzyme activity is not yet known, although it has been suggested that the promoter activity of this gene in the ancestors of higher primates was inactivated.

Methods

We used 5'-or 3'-RACE, GenomeWalking, reverse transcriptase polymerase chain reaction (RT-PCR) and dual Luciferase reporter assay for identification of the full-length cDNA, which includes the transcription initiation site and the promoter region of porcine alpha1,3GT gene.

Results

The region around exon 1 is guanine and cytosine

SUBMITTER: Koike C 

PROVIDER: S-EPMC2979301 | biostudies-literature | 2000 Nov

REPOSITORIES: biostudies-literature