Rapid transient production in plants by replicating and non-replicating vectors yields high quality functional anti-HIV antibody.

ABSTRACT:

Background

The capacity of plants and plant cells to produce large amounts of recombinant protein has been well established. Due to advantages in terms of speed and yield, attention has recently turned towards the use of transient expression systems, including viral vectors, to produce proteins of pharmaceutical interest in plants. However, the effects of such high level expression from viral vectors and concomitant effects on host cells may affect the quality of the recombinant product.

Methodology/principal findings

To assess the quality of antibodies transiently expressed to high levels in plants, we have expressed and characterised the human anti-HIV monoclonal antibody, 2G12, using both replicating and non-replicating systems based on deleted versions of Cowpea mosaic v

SUBMITTER: Sainsbury F 

PROVIDER: S-EPMC2980466 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature