Specific intronic p53 mutation in esophageal squamous cell carcinoma in Southern Thailand.

ABSTRACT:

Aim

To investigate p53 mutations in esophageal cancer in a high-risk population, and correlate them with smoking, alcohol consumption and betel chewing.

Methods

One hundred and sixty-five tumor samples of esophageal squamous cell carcinoma (ESCC) obtained from a university hospital in Songkhla province, Southern Thailand were investigated for p53 mutations in exons 5-8, using polymerase chain reaction-single strand conformation polymorphism analysis, followed by direct sequencing. A polymerase chain reaction-restriction fragment length polymorphism (RFLP) assay was additionally used to confirm possible germline mutation in intron 6. A history of risk habits was obtained by interviews. The association between risk habits and mutation frequency was evaluated using the χ(2) tes

SUBMITTER: Thongsuksai P 

PROVIDER: S-EPMC2980687 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature