EASY-HIT: HIV full-replication technology for broad discovery of multiple classes of HIV inhibitors.

ABSTRACT: HIV replication assays are important tools for HIV drug discovery efforts. Here, we present a full HIV replication system (EASY-HIT) for the identification and analysis of HIV inhibitors. This technology is based on adherently growing HIV-susceptible cells, with a stable fluorescent reporter gene activated by HIV Tat and Rev. A fluorescence-based assay was designed that measures HIV infection by two parameters relating to the early and the late phases of HIV replication, respectively. Validation of the assay with a panel of nine reference inhibitors yielded effective inhibitory concentrations consistent with published data and allowed discrimination between inhibitors of early and late phases of HIV replication. Finer resolution of the effects of reference drugs on different steps of HIV r

SUBMITTER: Kremb S 

PROVIDER: S-EPMC2981278 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature