Shotgun metabolomics approach for the analysis of negatively charged water-soluble cellular metabolites from mouse heart tissue.

ABSTRACT: A shotgun metabolomics approach using MALDI-TOF/TOF mass spectrometry was developed for the rapid analysis of negatively charged water-soluble cellular metabolites. Through the use of neutral organic solvents to inactivate endogenous enzyme activities (i.e., methanol/chloroform/H2O extraction), in conjunction with a matrix having minimal background noise (9-amnioacridine), a set of multiplexed conditions was developed that allowed identification of 285 peaks corresponding to negatively charged metabolites from mouse heart extracts. Identification of metabolite peaks was based on mass accuracy and was confirmed by tandem mass spectrometry for 90 of the identified metabolite peaks. Through multiplexing ionization conditions, new suites of metabolites could be ionized and "spectrometric isola

SUBMITTER: Sun G 

PROVIDER: S-EPMC2981504 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature