High sensitivity TSS prediction: estimates of locations where TSS cannot occur.

ABSTRACT:

Background

Although transcription in mammalian genomes can initiate from various genomic positions (e.g., 3'UTR, coding exons, etc.), most locations on genomes are not prone to transcription initiation. It is of practical and theoretical interest to be able to estimate such collections of non-TSS locations (NTLs). The identification of large portions of NTLs can contribute to better focusing the search for TSS locations and thus contribute to promoter and gene finding. It can help in the assessment of 5' completeness of expressed sequences, contribute to more successful experimental designs, as well as more accurate gene annotation.

Methodology

Using comprehensive collections of Cap Analysis of Gene Expression (CAGE) and other transcript data from mouse and human genomes, we

SUBMITTER: Schaefer U 

PROVIDER: S-EPMC2981523 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature