Comparison of metal and metal oxide media for phosphopeptide enrichment prior to mass spectrometric analyses.

ABSTRACT: Several affinity resins consisting of ionic metals or metal oxides were investigated for their phosphopeptide enrichment capabilities with subsequent mass spectrometric analyses. Commercially-available enrichment metal oxide affinity chromatography (MOAC) resins using manufacturer's and/or published protocols were compared and evaluated for the most efficient and selective method that could be implemented as a standard enrichment procedure. From these comparative analyses, using a tryptic digest of casein proteins, it was determined that in our hands, two of the resins out-performed the others based on a variety of criteria, including the number of phosphorylation sites identified during MS analyses, the lower numbers of nonspecifically bound peptides observed, and the limits of detection.

SUBMITTER: Gates MB 

PROVIDER: S-EPMC2982680 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature