RAId_aPS: MS/MS analysis with multiple scoring functions and spectrum-specific statistics.

ABSTRACT: Statistically meaningful comparison/combination of peptide identification results from various search methods is impeded by the lack of a universal statistical standard. Providing an E-value calibration protocol, we demonstrated earlier the feasibility of translating either the score or heuristic E-value reported by any method into the textbook-defined E-value, which may serve as the universal statistical standard. This protocol, although robust, may lose spectrum-specific statistics and might require a new calibration when changes in experimental setup occur. To mitigate these issues, we developed a new MS/MS search tool, RAId_aPS, that is able to provide spectrum-specific-values for additive scoring functions. Given a selection of scoring functions out of RAId score, K-score, Hyperscore

SUBMITTER: Alves G 

PROVIDER: S-EPMC2982831 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature