Perilipin overexpression in white adipose tissue induces a brown fat-like phenotype.

ABSTRACT:

Background

Perilipin A (PeriA) exclusively locates on adipocyte lipid droplets and is essential for lipid storage and lipolysis. Previously, we reported that adipocyte specific overexpression of PeriA caused resistance to diet-induced obesity and resulted in improved insulin sensitivity. In order to better understand the biological basis for this observed phenotype, we performed additional studies in this transgenic mouse model.

Methodology and principal findings

When compared to control animals, whole body energy expenditure was increased in the transgenic mice. Subsequently, we performed DNA microarray analysis and real-time PCR on white adipose tissue. Consistent with the metabolic chamber data, we observed increased expression of genes associated with fatty acid β-oxidat

SUBMITTER: Sawada T 

PROVIDER: S-EPMC2982838 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature