Synthetic peptide arrays for pathway-level protein monitoring by liquid chromatography-tandem mass spectrometry.

ABSTRACT: Effective methods to detect and quantify functionally linked regulatory proteins in complex biological samples are essential for investigating mammalian signaling pathways. Traditional immunoassays depend on proprietary reagents that are difficult to generate and multiplex, whereas global proteomic profiling can be tedious and can miss low abundance proteins. Here, we report a target-driven liquid chromatography-tandem mass spectrometry (LC-MS/MS) strategy for selectively examining the levels of multiple low abundance components of signaling pathways which are refractory to standard shotgun screening procedures and hence appear limited in current MS/MS repositories. Our stepwise approach consists of: (i) synthesizing microscale peptide arrays, including heavy isotope-labeled internal stand

SUBMITTER: Hewel JA 

PROVIDER: S-EPMC2984235 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature