Determination of the differentiation capacities of murines' primary mononucleated cells and MC3T3-E1 cells.

ABSTRACT:

Background

The main morphological features of primitive cells, such as stem and progenitor cells, are that these cells consists only one nucleus. The main purpose of this study was to determine the differentiation capacities of stem and progenitor cells. This study was performed using mononucleated cells originated from murine peripheral blood and MC3T3-E1 cells. Three approaches were used to determine their differentiation capacities: 1) Biochemical assays, 2) Gene expression analysis, and 3) Morphological observations.

Results

We found that both cells were able to differentiate into mature osteoblasts, as assayed by ALP activity. RT-PCR analysis showed the activation of the Opn gene after osteoblast differentiation. Morphological observations of both cells revealed the for

SUBMITTER: Yazid MD 

PROVIDER: S-EPMC2984396 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature