Dynamic, large-scale profiling of transcription factor activity from live cells in 3D culture.

ABSTRACT:

Background

Extracellular activation of signal transduction pathways and their downstream target transcription factors (TFs) are critical regulators of cellular processes and tissue development. The intracellular signaling network is complex, and techniques that quantify the activities of numerous pathways and connect their activities to the resulting phenotype would identify the signals and mechanisms regulating tissue development. The ability to investigate tissue development should capture the dynamic pathway activity and requires an environment that supports cellular organization into structures that mimic in vivo phenotypes. Taken together, our objective was to develop cellular arrays for dynamic, large-scale quantification of TF activity as cells organized into spherical struc

SUBMITTER: Weiss MS 

PROVIDER: S-EPMC2984444 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature