Decrypting the H-NS-dependent regulatory cascade of acid stress resistance in Escherichia coli.

ABSTRACT:

Background

H-NS regulates the acid stress resistance. The present study aimed to characterize the H-NS-dependent cascade governing the acid stress resistance pathways and to define the interplay between the different regulators.

Results

We combined mutational, phenotypic and gene expression analyses, to unravel the regulatory hierarchy in acid resistance involving H-NS, RcsB-P/GadE complex, HdfR, CadC, AdiY regulators, and DNA-binding assays to separate direct effects from indirect ones. RcsB-P/GadE regulatory complex, the general direct regulator of glutamate-, arginine- and lysine-dependent acid resistance pathways plays a central role in the regulatory cascade. However, H-NS also directly controls specific regulators of these pathways (e.g. cadC) and genes involved in gen

SUBMITTER: Krin E 

PROVIDER: S-EPMC2984483 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature