Intron 4 containing novel GABAB1 isoforms impair GABAB receptor function.

ABSTRACT:

Background

Gamma-aminobutyric acid type B (GABAB) receptors decrease neural activity through G protein signaling. There are two subunits, GABAB1 and GABAB2. Alternative splicing provides GABAB1 with structural and functional diversity. cDNA microarrays showed strong signals from human brain RNA using GABAB1 intron 4 region probes. Therefore, we predicted the existence of novel splice variants.

Methodology/principal findings

Based on the probe sequence analysis, we proposed two possible splice variants, GABAB1j and GABAB1k. The existence of human GABAB1j was verified by quantitative real-time PCR, and mouse GABAB1j was found from a microarray probe set based on human GABAB1j sequence. GABAB1j open reading frames (ORF) and expression patterns are not conserved across species,

SUBMITTER: Lee C 

PROVIDER: S-EPMC2987798 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature