Photounbinding of calmodulin from a family of CaM binding peptides.

ABSTRACT:

Background

Recent studies have shown that fluorescently labeled antibodies can be dissociated from their antigen by illumination with laser light. The mechanism responsible for the photounbinding effect, however, remains elusive. Here, we give important insights into the mechanism of photounbinding and show that the effect is not restricted to antibody/antigen binding.

Methodology/principal findings

We present studies of the photounbinding of labeled calmodulin (CaM) from a set of CaM-binding peptides with different affinities to CaM after one- and two-photon excitation. We found that the photounbinding effect becomes stronger with increasing binding affinity. Our observation that photounbinding can be influenced by using free radical scavengers, that it does not occur with

SUBMITTER: Neumuller KG 

PROVIDER: S-EPMC2987815 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature