High-throughput molecular identification of Staphylococcus spp. isolated from a clean room facility in an environmental monitoring program.

ABSTRACT:

Background

The staphylococci are one of the most common environmental isolates found in clean room facility. Consequently, isolation followed by comprehensive and accurate identification is an essential step in any environmental monitoring program.

Findings

We have used the API Staph identification kit (bioMérieux, France) which depends on the expression of metabolic activities and or morphological features to identify the Staphylococcus isolates. The API staphylococci showed low sensitivity in the identification of some species, so we performed molecular methods based on PCR based fingerprinting of glyceraldehyde-3-phosphate dehydrogenase encoding gene as useful taxonomic tool for examining Staphylococcus isolates.

Conclusions

Our results showed that PCR protocol use

SUBMITTER: Sheraba NS 

PROVIDER: S-EPMC2987874 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature