A multi-level study of recombinant Pichia pastoris in different oxygen conditions.

ABSTRACT:

Background

Yeasts are attractive expression platforms for many recombinant proteins, and there is evidence for an important interrelation between the protein secretion machinery and environmental stresses. While adaptive responses to such stresses are extensively studied in Saccharomyces cerevisiae, little is known about their impact on the physiology of Pichia pastoris. We have recently reported a beneficial effect of hypoxia on recombinant Fab secretion in P. pastoris chemostat cultivations. As a consequence, a systems biology approach was used to comprehensively identify cellular adaptations to low oxygen availability and the additional burden of protein production. Gene expression profiling was combined with proteomic analyses and the 13C isotope labelling based experimental de

SUBMITTER: Baumann K 

PROVIDER: S-EPMC2987880 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature