Quantification of the physiochemical constraints on the export of spider silk proteins by Salmonella type III secretion.

ABSTRACT:

Background

The type III secretion system (T3SS) is a molecular machine in gram negative bacteria that exports proteins through both membranes to the extracellular environment. It has been previously demonstrated that the T3SS encoded in Salmonella Pathogenicity Island 1 (SPI-1) can be harnessed to export recombinant proteins. Here, we demonstrate the secretion of a variety of unfolded spider silk proteins and use these data to quantify the constraints of this system with respect to the export of recombinant protein.

Results

To test how the timing and level of protein expression affects secretion, we designed a hybrid promoter that combines an IPTG-inducible system with a natural genetic circuit that controls effector expression in Salmonella (psicA). LacO operators are place

SUBMITTER: Widmaier DM 

PROVIDER: S-EPMC2987917 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature