Safety paradigm: genetic evaluation of therapeutic grade human embryonic stem cells.

ABSTRACT: The use of stem cells for regenerative medicine has captured the imagination of the public, with media attention contributing to rising expectations of clinical benefits. Human embryonic stem cells (hESCs) are the best model for capital investment in stem cell therapy and there is a clear need for their robust genetic characterization before scaling-up cell expansion for that purpose. We have to be certain that the genome of the starting material is stable and normal, but the limited resolution of conventional karyotyping is unable to give us such assurance. Advanced molecular cytogenetic technologies such as array comparative genomic hybridization for identifying chromosomal imbalances, and single nucleotide polymorphism analysis for identifying ethnic background and loss of heterozygosit

SUBMITTER: Stephenson E 

PROVIDER: S-EPMC2988272 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature