In vivo development of dendritic orientation in wild-type and mislocalized retinal ganglion cells.

ABSTRACT:

Background

Many neurons in the central nervous system, including retinal ganglion cells (RGCs), possess asymmetric dendritic arbors oriented toward their presynaptic partners. How such dendritic arbors become biased during development in vivo is not well understood. Dendritic arbors may become oriented by directed outgrowth or by reorganization of an initially unbiased arbor. To distinguish between these possibilities, we imaged the dynamic behavior of zebrafish RGC dendrites during development in vivo. We then addressed how cell positioning within the retina, altered in heart-and-soul (has) mutants, affects RGC dendritic orientation.

Results

In vivo multiphoton time-lapse analysis revealed that RGC dendrites initially exhibit exploratory behavior in multiple directions but

SUBMITTER: Choi JH 

PROVIDER: S-EPMC2988773 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature