Advanced glycation end products (AGEs) activate mast cells.

ABSTRACT:

Background and purpose

Advanced glycation endproducts (AGEs) represent one of the many types of chemical modifications that occur with age in long-lived proteins. AGEs also accumulate in pathologies such as diabetes, cardiovascular diseases, neurodegeneration and cancer. Mast cells are major effectors of acute inflammatory responses that also contribute to the progression of chronic diseases. Here we investigated interactions between AGEs and mast cells.

Experimental approaches

Histamine secretion from AGEs-stimulated mast cells was measured. Involvement of a receptor for AGEs, RAGE, was assessed by PCR, immunostaining and use of inhibitors of RAGE. Production of reactive oxygen species (ROS) and cytokines was measured.

Key results

Advanced glycation endproducts dose-

SUBMITTER: Sick E 

PROVIDER: S-EPMC2989594 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature