Sox10-Venus mice: a new tool for real-time labeling of neural crest lineage cells and oligodendrocytes.

ABSTRACT:

Background

While several mouse strains have recently been developed for tracing neural crest or oligodendrocyte lineages, each strain has inherent limitations. The connection between human SOX10 mutations and neural crest cell pathogenesis led us to focus on the Sox10 gene, which is critical for neural crest development. We generated Sox10-Venus BAC transgenic mice to monitor Sox10 expression in both normal development and in pathological processes.

Results

Tissue fluorescence distinguished neural crest progeny cells and oligodendrocytes in the Sox10-Venus mouse embryo. Immunohistochemical analysis confirmed that Venus expression was restricted to cells expressing endogenous Sox10. Time-lapse imaging of various tissues in Sox10-Venus mice demonstrated that Venus expression c

SUBMITTER: Shibata S 

PROVIDER: S-EPMC2989948 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature