Development of a real-time quantitative PCR assay for detection of a stable genomic region of BK virus.

ABSTRACT:

Background

BK virus infections can have clinically significant consequences in immunocompromised individuals. Detection and monitoring of active BK virus infections in certain situations is recommended and therefore PCR assays for detection of BK virus have been developed. The performance of current BK PCR detection assays is limited by the existence of viral polymorphisms, unknown at the time of assay development, resulting in inconsistent detection of BK virus. The objective of this study was to identify a stable region of the BK viral genome for detection by PCR that would be minimally affected by polymorphisms as more sequence data for BK virus becomes available.

Results

Employing a combination of techniques, including amino acid and DNA sequence alignment and interspeci

SUBMITTER: Iwaki KK 

PROVIDER: S-EPMC2989966 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature