Neuroprotective effects of andrographolide in a rat model of permanent cerebral ischaemia.

ABSTRACT:

Background and purpose

Andrographolide is a diterpenoid lactone isolated from a traditional medicinal herb, Andrographis paniculata. It possesses potent anti-inflammatory activity. The present study examined potential therapeutic effects of andrographolide on cerebral ischaemia using a rat model with permanent middle cerebral artery occlusion (pMCAO).

Experimental approach

The MCA in rats was permanently occluded (by cautery), and 24 h later neurological effects were assessed with behavioural scores. Infarct volume and microglial activation were determined histologically. The p65 form of the transcription factor, nuclear factor-κB (NF-κB), was measured by Western blot, and cytokines by immunoassay of brain extracts.

Key results

Andrographolide, given i.p. 1 h after pM

SUBMITTER: Chan SJ 

PROVIDER: S-EPMC2990163 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature