Dual-channel single-molecule fluorescence resonance energy transfer to establish distance parameters for RNA nanoparticles.

ABSTRACT: The increasing interest in RNA nanotechnology and the demonstrated feasibility of using RNA nanoparticles as therapeutics have prompted the need for imaging systems with nanometer-scale resolution for RNA studies. Phi29 dimeric pRNAs can serve as building blocks in assembly into the hexameric ring of the nanomotors, as modules of RNA nanoparciles, and as vehicles for specific delivery of therapeutics to cancers or viral infected cells. The understanding of the 3D structure of this novel RNA dimeric particle is fundamentally and practically important. Although a 3D model of pRNA dimer has been proposed based on biochemical analysis, no distance measurements or X-ray diffraction data have been reported. Here we evaluated the application of our customized single-molecule dual-viewing system f

SUBMITTER: Shu D 

PROVIDER: S-EPMC2990273 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature