Evidence of prokineticin dysregulation in fallopian tube from women with ectopic pregnancy.

ABSTRACT:

Objective

To demonstrate expression and regulation of prokineticins (PROKs) and their receptors (PROKRs) in fallopian tube (FT) from women who are not pregnant and women with ectopic pregnancy (EP).

Design

Tissue analysis.

Setting

Large United Kingdom teaching hospital.

Patient(s)

Women undergoing hysterectomy for benign gynecological conditions (n = 15) and surgery for EP (n = 16).

Intervention(s)

Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry were used to determine FT PROK/PROKR messenger RNA (mRNA) expression and protein localization, respectively. The PROK/PROKR levels were measured in tubal explant cultures stimulated with estrogen (E) and progestogen.

Main outcome measure(s)

Differential expres

SUBMITTER: Shaw JL 

PROVIDER: S-EPMC2990462 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature